Virus transmission by aphids

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Virus transmission by aphids

Virus transmission by aphids

Group leaders: Véronique Brault & Martin Drucker

The majority of plant viruses are transmitted from plant to plant by vectors, mostly aphids. In our lab, we study the interactions between plant, virus and aphid ultimately resulting in virus transmission. To conduct this project, different approaches are used as reverse genetics, transcriptomics, metabolomics and study of aphid behavior.

Objectives

     Projects

  • identify virus-induced deregulations in the host plant that can impact virus transmission by aphids
  • identify virus-induced deregulations in aphid vectors that can impact virus transmission
  • identify virus receptors in aphids

ANR ROME 2019-2022

Human Frontiers 2015-2019 

ANR Viraphiplant 2014-2018 

Two pathosystems

     Thesis & Postdoc

  • Turnip yellows virus (TuYV) which infects model or cultivated herbaceous plants. TuYV is transmitted by the aphid species Myzus persicae in a circulative and persistent mode. The virus goes through the intestinal epithelium and then invades the salivary gland cells before being released, together with saliva, during feeding. Virus is taken up by the aphid cells using virus-specific receptors.
  • Cauliflower mosaic virus (CaMV) which is, unlike TuYV, transmitted by M. persicae in a non-circulative and non-persistent manner meaning that it is retained externally at the aphid’s stylet tip.

These two models will enable us to identify the virus-induced deregulations in plants and aphids which are common or specific to either mode of transmission.

Quentin Chesnais (2019-2022)

David Halter (2016-2017)

Michaël Mulot (2014-2017)

Results

Identification of the first receptor of TuYV in M. persicae

TuYV-infected plants release more volatiles that attract aphids

A fluorescent polerovirus to follow virus movement in the plant

Mulot & Monsion 2018

Mulot & Monsion 2018, Front Microbiol
Claudel 2018

Claudel et al., 2018 IJMS
Boissinot 2018

Boissinot et al., 2017 Viruses

A fluorescent caulimovirus to study viral infection of plants

Transmission activation: a process also occuring for a potyvirus

Comparison of transmission activation of two viruses

Dader 2019

Dader et al., 2019 PloSOne
Berthelot 2019

Berthelot et al., 2019 Journal of Virology
Berthelot 2 2019

Berthelot et al., 2019 Scientific Reports

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Additional information

Read the article on The Conversation (French Version only)

Partners

  • CNRS-IBMP Strasbourg (Team of Véronique Ziegler-Graff)
  • INRA Montpellier (Team of Mickhail Pooggin)

Dans ce dossier

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Berthelot et al., 2019 Scientific reports: Schematic presentation of (left) the transmissible complexes of TuMV and CaMV bound to the stylets of an aphid vector (right).
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Mulot & Monsion 2018 Front Microbiol: The Ephrin receptor is involved in the acquisition of Turnip yellows virus in the intestinal cells of Myzus persicae.
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Graphical abstract: Claudel et al., 2018 IJMS: TuYV-infected Camelina emit more volatiles attracting aphids than infected Arabidopsis.
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Boissinot et al., 2017 Viruses: TuYV-GFP localization in an infected Camelina leaf. The fluorescent vessels represent sites of virus replication.
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Dader et al., 2019 PloSOne: Observation of CaMV in an infected Arabidopsis leaf. The P6 protein forms inclusions in infected cells.
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Berthelot et al., 2019 Journal of Virology: TuMV transmission model: Transmissible forms of TuMV, composed of virions and HC-Pro, just form when the aphid is present. Aphid puncture of the plant cell triggers calcium signaling and ROS synthesis, which in turn induces association of HC-Pro with virions yielding transmissible complexes.

Date de modification : 07 juin 2023 | Date de création : 10 avril 2019 | Rédaction : Inra Grand Est Colmar - Véronique Brault & Martin Drucker